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Cell Therapy with Embryonic Stem Cell-Derived Cardiomyocytes Encapsulated in Injectable Nanomatrix Gel Enhances Cell Engraftment and Promotes Cardiac Repair
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ban, Kiwon Park, Hun-Jun Kim, Sangsung Andukuri, Adinarayana Cho, Kyu-Won Hwang, Jung Wook Kim, Sang Yoon Kim, Woan-Sang Jun, Ho-Wook Yoon, Young-Sup |
| Copyright Year | 2014 |
| Abstract | A significant barrier to the therapeutic use of stem cells is poor cell retention in vivo. Here, we evaluate the therapeutic potential and long-term engraftment of cardiomyocytes (CMs) derived from mouse embryonic stem cells (mESCs) encapsulated in an injectable nanomatrix gel consisting of peptide amphiphiles incorporating cell adhesive ligand Arg-Gly-Asp-Ser (PA-RGDS) in experimental myocardial infarction (MI). We cultured rat neonatal CMs in PA-RGDS for 7 days and found that more than 90% of the CMs survived. Next, we intramyocardially injected mouse CM cell line HL-1 CMs with or without PA-RGDS into uninjured hearts. Histologic examination and flow cytometry analysis of digested heart tissues showed approximately 3-fold higher engraftment in the mice that received CMs with PA-RGDS compared to those without PA-RGDS. We further investigated the therapeutic effects and long-term engraftment of mESC-CMs with PA-RGDS on MI in comparison with PBS control, CM-only, and PA-RGDS only. Echocardiography demonstrated that the CM-only and CM+PA-RGDS groups showed higher cardiac function at week 2 compared to other groups. However, from 3 weeks, higher cardiac function was maintained only in the CM+PA-RGDS group; this was sustained for 12 weeks. Confocal microscopic examination of the cardiac tissues harvested at 14 weeks demonstrated sustained engraftment and integration of mESC-CMs into host myocardium in the CM+PA-RGDS group only. This study for the first time demonstrated that PA-RGDS encapsulation can enhance survival of mESC-derived CMs and improve cardiac function post-MI. This nanomatrix gel-mediated stem cell therapy can be a promising option for treating MI. |
| Starting Page | 10815 |
| Ending Page | 10825 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/nn504617g |
| PubMed reference number | 25210842 |
| Journal | Medline |
| Volume Number | 8 |
| Alternate Webpage(s) | https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/139104/1/T201406296.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/nn504617g |
| Journal | ACS nano |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
