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Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Toh, Wei Seong Guo, Xi-Min Chan, Jerry Kok Yen Yeow, Chen Hua Choo, Andre Boon Hwa Cao, Tong |
| Copyright Year | 2010 |
| Abstract | Human embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In this study, we explore the potential of these hESC-derived chondrogenic cells to produce an extracellular matrix (ECM)-enriched cartilaginous tissue construct when cultured in hyaluronic acid (HA)-based hydrogel, and further investigated the long-term reparative ability of the resulting hESC-derived chondrogenic cell-engineered cartilage (HCCEC) in an osteochondral defect model. We hypothesized that HCCEC can provide a functional template capable of undergoing orderly remodeling during the repair of critical-sized osteochondral defects (1.5 mm in diameter, 1 mm depth into the subchondral bone) in a rat model. In the process of repair, we observed an orderly spatial-temporal remodeling of HCCEC over 12 weeks into osteochondral tissue, with characteristic architectural features including a hyaline-like neocartilage layer with good surface regularity and complete integration with the adjacent host cartilage and a regenerated subchondral bone. By 12 weeks, the HCCEC-regenerated osteochondral tissue resembled closely that of age-matched unoperated native control, while only fibrous tissue filled in the control defects which left empty or treated with hydrogel alone. Here we demonstrate that transplanted hESC-derived chondrogenic cells maintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage tissue engineering. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.biomaterials.2010.05.064 |
| PubMed reference number | 20619789 |
| Journal | Medline |
| Volume Number | 31 |
| Issue Number | 27 |
| Alternate Webpage(s) | http://www.dentistry.nus.edu.sg/Research/files/FoD%20Publications%20in%20PubMed%2031Aug2010.pdf |
| Journal | Biomaterials |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
