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Recognition by anti-idiotypic anti-thyrotropin-releasing hormone (TRH) antibody of rat TRH receptors
| Content Provider | Semantic Scholar |
|---|---|
| Author | Satoh, Teturou Mori, Masatomo Murakami, Matsutaroh Iriuchijima, Tokuji Kobayashi, S. |
| Copyright Year | 1991 |
| Abstract | We asked whether anti-idiotypic antibodies raised against anti-TRH antibody could bind to TRH receptors in the rat anterior pituitary and brain. Six rabbits were immunized with IgG from a rabbit anti-TRH antiserum. One anti-idiotypic antibody caused strong, dose-dependent inhibition in anti-TRH antibody-binding to [125]I-TRH. This inhibition was not observed after treatment with goat anti-rabbit IgG antibody. The anti-idiotypic anti-TRH antibody significantly immunoprecipitated digitonin-solubilized pituitary TRH receptors. When eluates of digitonin-solubilized membranes which were adsorbed by either an anti-idiotypic anti-TRH IgG-, normal rabbit IgG-linked affinity column or control column were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and visualized by silver stain, only the former eluate showed two bands under nonreducing conditions; one corresponded to a molecular weight marker of 200K, the other to 100K. Western blotting analysis with an anti-idiotypic anti-TRH antiserum showed a single band of molecular weight 56K under reducing conditions. The present study indicates that one can make anti-idiotypic antibodies that specifically recognize TRH receptors. |
| Starting Page | 121 |
| Ending Page | 127 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0143-4179(91)90103-P |
| PubMed reference number | 1648676 |
| Journal | Medline |
| Volume Number | 18 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/014341799190103P |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/014341799190103P?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0143-4179%2891%2990103-P |
| Journal | Neuropeptides |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
