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GABAB receptor attenuation of GABAA currents in neurons of the mammalian central nervous system
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shen, Wen Shuang Nan, Changlong Nelson, Peter T. Ripps, Harris Slaughter, Malcolm M. |
| Copyright Year | 2017 |
| Abstract | Ionotropic receptors are tightly regulated by second messenger systems and are often present along with their metabotropic counterparts on a neuron's plasma membrane. This leads to the hypothesis that the two receptor subtypes can interact, and indeed this has been observed in excitatory glutamate and inhibitory GABA receptors. In both systems the metabotropic pathway augments the ionotropic receptor response. However, we have found that the metabotropic GABAB receptor can suppress the ionotropic GABAA receptor current, in both the in vitro mouse retina and in human amygdala membrane fractions. Expression of amygdala membrane microdomains in Xenopus oocytes by microtransplantation produced functional ionotropic and metabotropic GABA receptors. Most GABAA receptors had properties of α-subunit containing receptors, with ~5% having ρ-subunit properties. Only GABAA receptors with α-subunit-like properties were regulated by GABAB receptors. In mouse retinal ganglion cells, where only α-subunit-containing GABAA receptors are expressed, GABAB receptors suppressed GABAA receptor currents. This suppression was blocked by GABAB receptor antagonists, G-protein inhibitors, and GABAB receptor antibodies. Based on the kinetic differences between metabotropic and ionotropic receptors, their interaction would suppress repeated, rapid GABAergic inhibition. |
| Starting Page | 291 |
| Ending Page | 313 |
| Page Count | 23 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1015&context=pathology_facpub |
| PubMed reference number | 28348006 |
| Alternate Webpage(s) | https://doi.org/10.14814/phy2.13129 |
| DOI | 10.14814/phy2.13129 |
| Journal | Physiological reports |
| Volume Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amygdaloid structure CNS disorder Eye - Retina (MMHCC) GABA Receptor Ganglia Glutamic Acid Gonadotropin-Releasing Hormone Receptor In Vitro [Publication Type] Kinetics Mammals Neurons Plasma membrane Retinal Ganglion Cells Second Messenger Systems Subtype (attribute) Tissue membrane gamma-Aminobutyric Acid viral capsid secondary envelopment |
| Content Type | Text |
| Resource Type | Article |
