NDLI: Identification of Specific Plasma Proteins Determining the Agarose Gel Electrophoresis by the Immunosubtraction Technique

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Identification of Specific Plasma Proteins Determining the Agarose Gel Electrophoresis by the Immunosubtraction Technique

Content Provider	Semantic Scholar
Author	Merlini, Gianni Pavesi, Franco Carini, Angelo Zorzoli, Irene Valentini, Omar Aguzzi, Francesco
Copyright Year	1983
Abstract	The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, alpha-lipoproteins, alpha 1-antitrypsin, Gc-globulins, alpha 2-macroglobulin, haptoglobin, fibronectin, transferrin, beta-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
Starting Page	841
Ending Page	844
Page Count	4
File Format	PDF HTM / HTML
DOI	10.1515/cclm.1983.21.12.841
Alternate Webpage(s)	http://edoc.hu-berlin.de/oa/degruyter/cclm.1983.21.12.841.pdf
PubMed reference number	6663244
Alternate Webpage(s)	https://doi.org/10.1515/cclm.1983.21.12.841
Journal	Medline
Volume Number	21
Journal	Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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