NDLI: Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis.

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Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis.

Content Provider	Semantic Scholar
Author	Martín-Parras, L. Lucas, Isabelle Martínez-Robles, María Luisa Hernandez, Pablo Ayala Hyrien, Olivier Schvartzman, Jorge B.
Copyright Year	1998
Abstract	Neutral/neutral two-dimensional (2D) agarose gelelectrophoresis was used to investigate populations of the different topological conformations that pBR322 can adopt in vivo in bacterial cells as well as in Xenopus egg extracts. To help in interpretation and identification of all the different signals, undigested as well as DNA samples pretreated with DNase I, topoisomerase I and topoisomerase II were analyzed. The second dimension of the 2D gel system was run with or without ethidium bromide to account for any possible changes in the migration behavior of DNA molecules caused by intercalation of this planar agent. Finally, DNA samples were isolated from a recA-strain of Escherichia coli , as well as after direct labeling of the replication intermediates in extracts of Xenopus laevis eggs. Altogether, the results obtained demonstrated that 2D gels can be readily used to identify most of the complex topological populations that circular molecules can adopt in vivo in both bacteria and eukaryotic cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://digital.csic.es/bitstream/10261/47837/1/nar-1998.pdf
PubMed reference number	9649629v1
Volume Number	26
Issue Number	14
Journal	Nucleic acids research
Language	English
Access Restriction	Open
Subject Keyword	Agarose Gel Deoxyribonuclease I Electrophoresis, Agar Gel Ethidium Bromide Gel Electrophoresis (lab technique) Ovum Topoisomerase II
Content Type	Text
Resource Type	Article

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