Loading...
Please wait, while we are loading the content...
Similar Documents
The complete nucleotide sequence of turnip mosaic potyvirus RNA.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nicolas, Olaf Laliberté, J. F. |
| Copyright Year | 1992 |
| Abstract | The complete RNA genome of turnip mosaic potyvirus (TuMV) was amplified by seven consecutive reverse transcriptase-polymerase chain reactions and cloned into pUC9. The viral RNA is 9830 nucleotides long and contains a single open reading frame (ORF) of 9489 bases encoding a large polyprotein of 3863 amino acids with a calculated M(r) of 358,000. The non-coding region (NCR) preceding the ORF is 129 nucleotides long and has a high AU content (70%). Its predicted secondary structure is characterized by a hairpin loop with a free energy loss of -69.9 kJ/mol. The termination codon is followed by an AU-rich NCR of 209 bases, excluding the poly(A) tail. Seven potential nuclear inclusion a proteinase (NIa-Pro) recognition heptapeptides are found in the polyprotein. Their sequences agree with consensus potyviral NIa-Pro cleavage sequences except for that at the 6K-VPg site, which is characterized by a glutamic acid residue preceding the hydrolysed peptide bond. The TuMV proteins are similar to their corresponding potyviral proteins. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/0022-1317-73-11-2785 |
| PubMed reference number | 1431807 |
| Journal | Medline |
| Volume Number | 73 |
| Part | 11 |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/jgv/73/11/JV0730112785.pdf?accname=guest&checksum=75D5317B4D73B45FCFCEE4E169AE16AB&expires=1545371235&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/73/11/JV0730112785.pdf?accname=guest&checksum=F02089F662888B2CFC8FDCEF507848AB&expires=1541732098&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/73/11/JV0730112785.pdf?accname=guest&checksum=B5E2B8C9F418A4410317930D1CA24B8F&expires=1543313803&id=id |
| Alternate Webpage(s) | https://doi.org/10.1099/0022-1317-73-11-2785 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |