NDLI: The complete nucleotide' sequence of turnip mosaic potyvirus RNA (1992)

Please wait, while we are loading the content...

The complete nucleotide' sequence of turnip mosaic potyvirus RNA (1992)

Content Provider	CiteSeerX
Author	Nicolas, Olivier Lalibert, Jean-Frangois
Abstract	The complete RNA genome of turnip mosaic potyvirus (TuMV) was amplified by seven consecutive reverse transcriptase-polymerase chain reactions and cloned into pUC9. The viral RNA is 9830 nucleotides long and contains a single open reading frame (ORF) of 9489 bases encoding a large polyprotein of 3863 amino acids with a calculated Mr of 358 000. The non-coding region (NCR) preceding the ORF is 129 nucleotides long and has a high AU content (70%). Its predicted secondary structure is characterized by a hairpin loop with a free energy loss o f-69.9 kJ/mol. The termination codon is followed by an AU-rich NCR of 209 bases, excluding the poly(A) tail. Seven potential nuclear inclusion a proteinase (NIa-Pro) recognition heptapeptides are found in the polyprotein. Their sequences agree with consensus potyviral NIa-Pro cleavage sequences except for that at the 6K-VPg site, which is characterized by a glutamic acid residue preceding the hydrolysed peptide bond. The TuMV proteins are similar to their corresponding potyviral proteins.
File Format	PDF
Journal	Journal of General Virology
Language	English
Publisher Date	1992-01-01
Access Restriction	Open
Subject Keyword	Complete Nucleotide Turnip Mosaic Potyvirus Rna Amino Acid Tumv Protein Large Polyprotein Hydrolysed Peptide Bond Non-coding Region Kj Mol Single Open Reading Frame Recognition Heptapeptides Au-rich Ncr Consecutive Reverse Transcriptase-polymerase Chain Reaction Turnip Mosaic Potyvirus High Au Content Corresponding Potyviral Protein Seven Potential Nuclear Inclusion Glutamic Acid Termination Codon Free Energy Loss Predicted Secondary Structure 6k-vpg Site Hairpin Loop Viral Rna Complete Rna Genome Consensus Potyviral Nia-pro Cleavage Sequence Calculated Mr
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in