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Caracterização molecular de estirpes de rotavírus em rebanhos bovinos leiteiros e de corte das regiões Nordeste e Centro-oeste no Estado de São Paulo
| Content Provider | Semantic Scholar |
|---|---|
| Author | Salles, R. F. De M. |
| Copyright Year | 2009 |
| Abstract | The present study aimed to determine the Group A rotavirus and G/P genotyping in detected virus strains in dairy and beef cattle calves from July 2006 to September 2008 in Sao Paulo State-Brazil farms. 395 calves in 18 flocks (9 dairy cattle and 9 beef cattle) from 1 to 60-day-old were sampled, independently whether manifesting clinically diarrhea or not. By using Poliacrylamide Gel Electrophoresis Technique (PAGE), the rotavirus occurrence of 33.3% (06/18) among flocks and 8.6% (34/395) among the population sampled were determined. The higher infection frequency was detected in 16-to-30-day-old calves (P<0.01). Rotavirus-infected flocks rotavirus-infected were diagnosticated as much the ones having clinical symptoms of diarrhea (22%, 29/133) as the ones clinically normal (1.9%, 05/262), shawing a correlation between the presence of infection and the diarrhea manifestation (p<0.01). Related to the kind of exploration, the occurrence in dairy cattle was 5.3% (14/264) while in the beef cattle the occurrence was higher (15.3%, 20/131). The rotavirus genome profile analysis by PAGE identified two distinct electroferotypes, in which differences were located in the following segment migration positions: 2, 3, 7, 8 and 9. The genotyping of the Rotavirus sample by polymerase chain reaction (RT-snmPCR) revealed that the circulating strains among the flocks were G6P[5], G10P[5] e G?P[1]. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.fcav.unesp.br/Home/download/pgtrabs/mvp/m/3695.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
