Loading...
Please wait, while we are loading the content...
Similar Documents
Quantitative autoradiography of [3H]prazosin binding sites in rat forebrain
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rainbow, Thomas C. Biegon, Anat |
| Copyright Year | 1983 |
| Abstract | We have used the LKB Ultrofilm method of autoradiography to localize and quantify in rat forebrain the binding sites for [3H]prazosin, a highly-selective antagonist for the alpha 1 adrenoreceptor subtype. Frozen 32 microns thick brain sections were labeled in vitro with 1 nM [3H]prazosin and applied against LKB Ultrofilm for 60 days to generate autoradiograms. Non-specific binding was defined as the labeling in the presence of 10 microM phentolamine. The highest levels of prazosin binding were found in layer V of the motor portion of the frontoparietal cortex and in all nuclei of the thalamus. Moderate levels of alpha 1 receptors were observed in the remaining layers of the cerebral cortex and in most regions of the limbic system. Low levels of prazosin binding occurred in the caudate-putamen and the accumbens nucleus. Our results indicate that alpha 1 adrenoceptors are distributed heterogeneously throughout the rat forebrain. |
| Starting Page | 221 |
| Ending Page | 226 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0304-3940(83)90042-3 |
| PubMed reference number | 6316208 |
| Journal | Medline |
| Volume Number | 40 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0304394083900423 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0304394083900423?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0304-3940%2883%2990042-3 |
| Journal | Neuroscience Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
