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Surface plasmon resonance detection for capillary electrophoresis separations.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Whelan, Rebecca J. Zare, Richard N. |
| Copyright Year | 2003 |
| Abstract | A miniaturized surface plasmon resonance sensor has been used as an on-line detector for capillary electrophoresis separations. The capillary was modified slightly to shield the sensor electronics from the high voltages applied during the separation. A three-component mixture of high refractive index materials was separated and detected at the millimolar level by an untreated gold-sensing surface. A simple protein immobilization procedure was used to functionalize the surface for selective protein detection. A hybrid buffer system was developed, in which both the deposition of immobilized protein layers and the electrophoretic delivery of protein analytes were optimized. The detection system has a reproducibility of 15%, a dynamic range of 3 orders of magnitude, and a detection limit for IgG of 2 fmol. |
| Starting Page | 510 |
| Ending Page | 512 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://web.stanford.edu/group/Zarelab/publinks/700.pdf |
| Alternate Webpage(s) | http://www.harvardapparatus.com/media/harvard/pdf/PP159.pdf |
| PubMed reference number | 12659220v1 |
| Volume Number | 75 |
| Issue Number | 6 |
| Journal | Analytical chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affinity Bands Buffers Capillary vessel Chemistry Techniques, Analytical Customize Detectors Dynamic Range Electrophoresis Electrophoresis, Capillary Entity Name Part Qualifier - adopted Femtomole Heating Joule, Unit of energy Millimole per Liter Preparation Shield Device Component Surface Plasmon Resonance Thermostat Device Component analyte anatomical layer orders - HL7PublishingDomain |
| Content Type | Text |
| Resource Type | Article |
