Capillary electrophoretic separation of proteins and peptides by ion-pairing with heptanesulfonic acid.

Content Provider	Semantic Scholar
Author	Charvátová, Jana Eckhardt, Adam Cserháti, Tibor Forgács, Esther Deyl, Zdeněk
Copyright Year	2004
Abstract	Heptanesulfonic acid as ion-pairing agent was used for the separation of mixtures of low and high molecular mass peptides/proteins by capillary electrophoresis. The separation conditions used were: capillary 37 cm (30 cm to the detector) x 75 microm i.d., voltage 10 kV, phosphate buffer 50 mmol/l, ion-pairing agent heptanesulfonic acid at three different concentrations, namely, 0, 20 or 100 mmol/l, pH 2.5. The separation reflected the ion-pairing equilibria between the ion-pairing agent and the peptide/protein analytes. The influence of ion-pairing on sample mobility (running time) was more pronounced in case of the higher-molecular peptides as compared to the low molecular ones. This difference offers the possibility to separate low and high molecular peptides/proteins that under the absence of the ion-pairing agent would co-migrate. The principle of this approach was demonstrated on a randomly selected set of peptides/proteins; the practical applicability was demonstrated on a set of CNBr peptides arising from a naturally occurring mixture of collagen types I and III.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.analyt.natureblink.com/publikace/ion-pairing800.pdf
PubMed reference number	14698250v1
Volume Number	800
Issue Number	1-2
Journal	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Language	English
Access Restriction	Open
Subject Keyword	Capillary vessel Cyanogen Bromide Czech dysplasia, metatarsal type Detectors Electrophoresis Electrophoresis, Capillary Ions Iontophoresis Millimole per Liter Molecular Mass PHOSPHATE BUFFERS Thioctic Acid analyte funding grant inorganic phosphate voltage
Content Type	Text
Resource Type	Article