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Thin Layer Chromatography (TLC) and Phytochemical Analysis of Moringa Oleifera Methanol, Ethanol, Water and Ethyl Acetate Extracts
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shaeroun, Ahlam Rashed Ameerah Alqamoudy, Ahmed Belgasem Ahmed Hamed Mohamed, Khalifa. S. Kushlaf, Nadea Almunir Nouri Misbah, A.m.el-Mahmoudy Akram Almabrouk Almes, Seham Tm Oshkondali Zuhur Rajab |
| Copyright Year | 2019 |
| Abstract | Many medicinal plants were studied for their phytochemical contents. Moringa oleifera is one of these plants. It is widely distributed in tropical and subtropical areas. It has an important medical uses with high nutritional value. This study, primarily aimed to carry out a preliminary phytochemical screening to detect the major classes of bioactive compounds presented in Moringa oleifera dry leaves. Many solvents (Methanol, ethanol, ethanol acetate and water) used to determine the best solvent can be used for extraction and to perform thin layer chromatography (TLC) profiling of all sequential extracts. TLC was carried out in silica gel plates using many mobile phase protocols, chloroform: methanol: ethanol (1:1:1), (2:2:0.5) and Chloroform: Glacial acetic acid: methanol (4:5:1) and use four mentioned solvents. Phytochemical screening of four extracts showed the presence of alkaloids, flavonoids, tannins, and phenols in all solvent extracts. However, the concentration of these compounds is more with ethanol and ethanol acetate extracts (+++). As well as thin layer chromatographic studies of the Moringa olifera leave extract (methanol, ethanol, ethanol acetate and water) constituted different colored phytochemical compounds with different Rf values. Methanol and ethyl acetate extracts were the best which show 4 bands with Rf less than 1 while with ethanol extract 5 bands with Rf less than 1 and with water extract no band appeared by using chloroform: methanol: ethanol (1:1:1). Furthermore with the other mobile phases detect fewer bands with different solvent extracts. The results obtained in the present study revealed that the concentration of the bioactive compounds of Moringa oleifera leaves extracts by using ethanol and ethyl acetate as a solvent is more than the other solvents. On the other hand, TLC showed 4 bands with better Rf by using methanol and ethyl acetate extract. Conclusion, the methanol and ethyl acetate extracts for Moringa oleifer leaves contain a higher content of bioactive compounds, which can be used for further researches on this plant. |
| Starting Page | 817 |
| Ending Page | 820 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.36348/sjmps.2019.v05i10.002 |
| Volume Number | 05 |
| Alternate Webpage(s) | https://saudijournals.com/media/articles/SJMPS_510_817-820_c.pdf |
| Alternate Webpage(s) | https://doi.org/10.36348/sjmps.2019.v05i10.002 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
