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A novel colorimetric potassium sensor based on the substitution of lead from G-quadruplex.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sun, Huijiao Li, Xiaohong Li, Yunchao Fan, Louzhen Kraatz, Heinz-Bernhard |
| Copyright Year | 2013 |
| Abstract | Potassium ions play diverse roles in biological processes, and abnormal K(+) levels are the hallmarks of diseases. However, the potential clinical application of the developed DNA-based K(+) sensors remains a challenge due to the presence of Pb(2+) in blood samples. In this contribution, a novel colorimetric potassium sensing assay that functions in the presence of Pb(2+) is reported. This approach is based on conformational switching of a hairpin DNA structure to a G-quadruplex. Specifically, the hairpin DNA containing G-rich aptamer T30695 is exposed to successive amounts of Na(+), Pb(2+) and K(+). These cations induce formation of the corresponding metal-stabilized G-quadruplex, which acts as DNAzyme (with hemin as a cofactor) for the catalytic oxidation of ABTS by H(2)O(2). Importantly, studies presented here show that K(+) replaces Pb(2+) from the G-quadruplex to form K(+)-stabilized G-quadruplex, which differ in the catalytic behavior. With Pb(2+)-stabilized G-quadruplex as a probe, a highly sensitive and selective colorimetric detection of K(+) is achieved in the presence of Pb(2+) and excessive Na(+) (140 mM) with the detection limit of 1.9 nM. This system represents the first known DNAzyme-based colorimetric K(+) sensor, which works in the presence of Pb(2+). Finally, the sensor is successfully applied for K(+) detection in a real human serum sample containing Pb(2+). |
| Starting Page | 115 |
| Ending Page | 123 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/an/c2/c2an36564b/c2an36564b.pdf |
| PubMed reference number | 23211869v1 |
| Alternate Webpage(s) | https://doi.org/10.1039/c2an36564b |
| DOI | 10.1039/c2an36564b |
| Journal | The Analyst |
| Volume Number | 138 |
| Issue Number | 3 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cations DNA, Catalytic G-quadruplex DNA formation Hemin Ions Potassium T 30695 aptamer chemical cofactor oxidation |
| Content Type | Text |
| Resource Type | Article |
