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Simultaneous mechanical loading and confocal reflection microscopy for three-dimensional microbiomechanical analysis of biomaterials and tissue constructs.

Content Provider	Semantic Scholar
Author	Voytik-Harbin, Sherry L. Kokini, Klod
Copyright Year	2003
Abstract	At present, mechanisms by which specific structural and mechanical properties of the three-dimensional extracellular matrix microenvironment influence cell behavior are not known. Lack of such knowledge precludes formulation of engineered scaffolds or tissue constructs that would deliver specific growth-inductive signals required for improved tissue restoration. This article describes a new mechanical loading-imaging technique that allows investigations of structural-mechanical properties of biomaterials as well as the structural-mechanical basis of cell-scaffold interactions at a microscopic level and in three dimensions. The technique is based upon the integration of a modified, miniature mechanical loading instrument with a confocal microscope. Confocal microscopy is conducted in a reflection and/or fluorescence mode for selective visualization of load-induced changes to the scaffold and any resident cells, while maintaining each specimen in a "live," fully hydrated state. This innovative technique offers several advantages over current biomechanics methodologies, including simultaneous visualization of scaffold and/or cell microstructure in three dimensions during mechanical loading; quantification of macroscopic mechanical parameters including true stress and strain; and the ability to perform multiple analyses on the same specimen. This technique was used to determine the structural-mechanical properties of three very different biological materials: a reconstituted collagen matrix, a tissue-derived biomaterial, and a tissue construct representing cells and matrix.
Starting Page	74
Ending Page	85
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.cyto.purdue.edu/archive/flowcyt/research/pdfs/2003/harbin-2003.pdf
Alternate Webpage(s)	http://www.cyto.purdue.edu/archive/flowcyt/research/pdfs/2005/Simultaneous_Mechanical_Loading_and_Confocal.pdf
Alternate Webpage(s)	http://www.cyto.purdue.edu/flowcyt/research/pdfs/2005/Simultaneous_Mechanical_Loading_and_Confocal.pdf
Alternate Webpage(s)	http://www.cyto.purdue.edu/flowcyt/research/pdfs/harbin-2003.pdf
PubMed reference number	12597789v1
Volume Number	9
Issue Number	1
Journal	Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Language	English
Access Restriction	Open
Subject Keyword	Biomaterials CDISC SEND Biospecimens Terminology Dimensions Extracellular Matrix Fluorescence Imagery Imaging Techniques Microscope Device Component Microscopy, Confocal Quantitation
Content Type	Text
Resource Type	Article

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Simultaneous Mechanical Loading and Confocal Reflection Microscopy for Three-Dimensional Microbiomechanical Analysis of Biomaterials and Tissue Constructs

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Simultaneous Mechanical Loading and Confocal Reflection Microscopy for Three-Dimensional Microbiomechanical Analysis of Biomaterials and Tissue Constructs

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