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Selective Detection and Quanti fi cation of Modi fi ed DNA with Solid-State Nanopores Autumn
| Content Provider | Semantic Scholar |
|---|---|
| Author | Carlsen, Tim Zahid, Osama K. Ruzicka, Jan A. Taylor, Ethan Will Hall, Adam R. |
| Copyright Year | 2014 |
| Abstract | We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein−DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://docs.wixstatic.com/ugd/046053_d33ec4711e5c4fd9958ae0dbab3eed27.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
