NDLI: Pectenotoxin-2 induces G2/M phase cell cycle arrest in human breast cancer cells via ATM and Chk1/2-mediated phosphorylation of cdc25C.

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Pectenotoxin-2 induces G2/M phase cell cycle arrest in human breast cancer cells via ATM and Chk1/2-mediated phosphorylation of cdc25C.

Content Provider	Semantic Scholar
Author	Moon, Dong-Oh Kim, Mun-Ock Kim, Se-Kwon Choi, Young Hyun Kim, Gi-Young
Copyright Year	2010
Abstract	Although pectenotoxin-2 (PTX-2) is known to regulate the actin depolymerization and to induce apoptosis through downregulation of telomerase activity, little is known on its effect on the cell cycle regulation. Therefore, we investigated the effects of PTX-2 on G2/M arrest in human breast cancer cells (MDA-MB-231 and MCF-7). Treatment with PTX-2 significantly suppressed cell proliferation and induced G2/M phase arrest through down-regulation of cyclin B1 and cdc2 expression, but also through phosphorylation of cdc25C. We found increased phosphorylation of ATM and Chk1/2 in a PTX-2 dose-dependent manner. Furthermore, treatment with PTX-2 increased H2O2 generation with correlated G2/M arrest. Our results showed that ATM- and Chk1/2-mediated phosphorylation of cdc25C plays a major role in G2/M arrest, but not in H2O2 generation induced by PTX-2 treatment. We also observed that PTX-2-induced cell cycle arrest was not restricted to p53 status in human breast cancer cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/or/24/1/271/download
PubMed reference number	20514472v1
Volume Number	24
Issue Number	1
Journal	Oncology reports
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Breast Cancer Cell CDC25C gene Cardiac Arrest Cell Cycle Arrest Cell Cycle Control Cell Proliferation Down-Regulation Hydrogen Peroxide Mammary Neoplasms Negative Regulation of Mitosis Telomerase Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation actin filament depolymerization ammonium tetrathiomolybdate
Content Type	Text
Resource Type	Article

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