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Immunolocalization of subtype 2 angiotensin II (AT2) receptor protein in rat heart.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Zheng-Huan Moore, A. F. Ozono, Ryoji Siragy, Helmy M. |
| Copyright Year | 1998 |
| Abstract | Angiotensin II exerts its effects on cardiovascular function and water and sodium homeostasis by interacting with plasma membrane receptors on target organs. The existence of subtype 2 angiotensin II (AT2) receptors in the rat heart has been demonstrated by ligand binding and reverse transcription-polymerase chain reaction. In the present study, the expression and localization of AT2 receptor protein in the rat heart was investigated using an antipeptide polyclonal antibody against the native rat AT2 receptor by light microscopic immunocytochemistry and Western blot analysis. In frozen tissue sections, positive immunostaining was observed in the myocardium and coronary vessels throughout the ventricle and atrium of neonatal and young rat hearts. Coronary vessels of the neonatal heart were more intensely stained compared with the surrounding myocardium. Positive immunoreactivity in the coronary vessels of young rats was localized to vascular endothelium but not in the smooth muscle cells. Preadsorption controls were all negative. Western blot analysis showed that the AT2 receptor protein (approximately 44 kDa) was detectable from the AT2 receptor-transfected COS-7 cells and neonatal rat cardiac myocytes but not from fibroblasts or young rat aortic smooth muscle cells. The neonatal rat heart expressed significantly more AT2 receptors than young rat heart. These data provide the first direct evidence for the expression and localization of AT2 receptor protein in the rat heart. |
| Starting Page | 78 |
| Ending Page | 83 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://hyper.ahajournals.org/content/hypertensionaha/32/1/78.full.pdf?download=true |
| Alternate Webpage(s) | http://hyper.ahajournals.org/content/hypertensionaha/32/1/78.full.pdf |
| PubMed reference number | 9674641v1 |
| Volume Number | 32 |
| Issue Number | 1 |
| Journal | Hypertension |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Angiotensin II Receptor, Type 2 Angiotensins Blood Vessel Cardiovascular Diseases Coronary Vessels Endothelium Endothelium, Vascular Heart Ventricle Immunocytochemistry Ligands Muscle Cells Myocardium Myocytes, Cardiac Myocytes, Smooth Muscle Organ Plasma membrane Polymerase Chain Reaction Reverse Transcription Smooth muscle (tissue) Sodium Western Blot physical hard work polyclonal antibody receptor |
| Content Type | Text |
| Resource Type | Article |
