Angiotensin II AT(2) receptor inhibits smooth muscle cell migration via fibronectin cell production and binding.

Content Provider	Semantic Scholar
Author	Chassagne, Catherine Adamy, Christophe Ratajczak, Philippe Gingras, Bruno Teiger, Emmanuel Planus, Emmanuelle Oliviéro, Patricia Rappaport, Lydie Samuel, Jane-Lise Meloche, Sylvain
Copyright Year	2002
Abstract	To explore the vascular function of the angiotensin II (ANG II) AT(2) receptor subtype (AT(2)R), we generated a vascular smooth muscle cell (SMC) line expressing the AT(2)R (SMC-vAT(2)). The involvement of AT(2)R in the motility response of SMCs was examined in SMC-vAT(2) cells and their controls (SMC-v) cultured on either laminin or fibronectin matrix proteins with the agarose drop technique. All experiments were conducted in the presence of a saturating concentration of losartan to inactivate the AT(1)R subtype. Under basal conditions, both cell lines migrated outside drops, but on laminin only. Treatment with ANG II significantly inhibited the migration of SMC-vAT(2) but not SMC-v cells, and this effect was prevented by the AT(2)R antagonist CGP-42112A. The decreased migration of SMC-vAT(2) was not associated with changes in cell growth, cytoskeleton stiffness, or smooth muscle actin, desmin, and tenascin expression. However, it was correlated with increased synthesis and binding of fibronectin. Both responses were prevented by incubation with selective AT(2)R antagonists. Addition of GRGDTP peptide, which prevents cell attachment of fibronectin, reversed the AT(2)R inhibitory effect on SMC-vAT(2) migration. These results suggest that activated ANG II AT(2)R inhibits SMC migration via cellular fibronectin synthesis and associated cell binding.
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Alternate Webpage(s)	http://ajpcell.physiology.org/content/ajpcell/282/4/C654.full.pdf
Alternate Webpage(s)	http://ajpcell.physiology.org/content/ajpcell/282/4/c654.full.pdf
Alternate Webpage(s)	http://ajpcell.physiology.org/content/early/2001/11/27/ajpcell.00318.2001.full.pdf
PubMed reference number	11880254v1
Volume Number	282
Issue Number	4
Journal	American journal of physiology. Cell physiology
Language	English
Access Restriction	Open
Subject Keyword	Angiotensin II Angiotensins Cell-Matrix Junction Cultured Cell Line IGF2R protein, human Laminin Losartan Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Saturated Smooth muscle (tissue) cell growth
Content Type	Text
Resource Type	Article