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Intercellular Transfer and Supramolecular Organization of Human Leukocyte Antigen C at Inhibitory Natural Killer Cell Immune Synapses 〉
| Content Provider | Semantic Scholar |
|---|---|
| Author | Eleme, Konstantina McCann, Fiona E. |
| Copyright Year | 2001 |
| Abstract | After accumulation of target cell human leukocyte antigen (HLA)-C at inhibitory natural killer (NK) cell immune synapses, some HLA-C transfers from target cells to NK cell plasma membranes and cytoplasm. This unexpected intercellular transfer of HLA-C is dependent on NK receptor recognition, since HLA-Cw6 or -Cw4 but not -Cw3 transfer to an NK transfectant expressing killer Ig-like receptor (KIR)2DL1. Strikingly, live-cell time-lapse laser scanning confocal microscopy shows vesicles containing target cell green fluorescent protein-tagged HLA-C migrating away from immune synapses into NK cells. Unlike clustering of HLA-C at the immune synapse, intercellular transfer of HLA-C is dependent on NK cell ATP, but not target cell ATP. However, the intercellular transfer of HLA-C is not dependent on active polymerization of the actin cytoskeleton. In addition, different arrangements of HLA-C are seen at inhibitory NK immune synapses, and these alter as NK synapses mature, but in a fashion distinct from that seen upon T cell activation. |
| Starting Page | 1507 |
| Ending Page | 1517 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 11714757 |
| Volume Number | 194 |
| Journal | The Journal of experimental medicine |
| Alternate Webpage(s) | http://jem.rupress.org/content/jem/194/10/1507.full.pdf |
| Alternate Webpage(s) | http://spiral.imperial.ac.uk/bitstream/10044/1/17914/2/Intercellular%20transfer%20and%20supramolecular%20organization%20of%20human%20leukocyte%20antigen%20C%20at%20inhibitory%20natural%20killer%20cell%20immune%20synapses..pdf |
| Alternate Webpage(s) | http://jem.rupress.org/content/194/10/1507.full.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/54/00/011232.PMC2193674.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chronic Lymphocytic Leukemia Green Fluorescent Proteins HLA Antigens HLA-C Antigens HLA-DQ Antigens Histocompatibility Antigens Class II Leukemia, B-Cell Leukocytes Microscopy, Confocal Microscopy, Confocal, Laser Scanning Natural Killer Cells Plasma Cells Plasma membrane Synapses Tracer Vesicle (morphologic abnormality) |
| Content Type | Text |
| Resource Type | Article |
