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Production and characterization of endo-β-1,4- glucanase from thermophilic fungus
| Content Provider | Semantic Scholar |
|---|---|
| Author | Raza, Muhammad Asam Shafiq-Ur-Rehman |
| Copyright Year | 2009 |
| Abstract | Chaetomium thermophile produces substantial extra-cellular cellulase on several cellulosic substrates (wheat straw, wheat bran and corn straw etc.) including simple sugars. 2% (w/v) wheat straw, wheat bran and corn straw were used as carbon source and urea was used as a nitrogen source. Experiment was conducted in a 20-l stirred tank reactor under the following conditions: pH 5.0, stirring speed 300 rpm, temperature 45 o C and aeration 50% (DO). The samples were withdrawn after every 12 h regularly and enzyme activity was assayed. Endo-β-1,4-glucanase activities on wheat straw, wheat bran and corn straw were 2.51, 1.62 and 5.10 U/ml, respectively and extra-cellular protein contents were 1.48, 1.17 and 2.88 mg/ml, respectively. Specific activity of enzyme after ammonium sulphate precipitation and Fast Protein Liquid Chromatography (FPLC) was 2.1 and 3.3 U/mg of protein, respectively. SDS-PAGE showed only a single band at 53 kDa. The optimum temperature and pH of the CMCase were 60 o C and from 6.0 - 7.5, respectively. |
| Starting Page | 3297 |
| Ending Page | 3302 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.5897/AJB2009.000-9326 |
| Volume Number | 8 |
| Alternate Webpage(s) | https://www.ajol.info/index.php/ajb/article/download/61084/49277 |
| Alternate Webpage(s) | https://doi.org/10.5897/AJB2009.000-9326 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
