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Roles of the CDK phosphorylation sites of yeast Cdc6 in chromatin binding and rereplication.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Honey, Sangeet Futcher, Bruce |
| Copyright Year | 2007 |
| Abstract | The Saccharomyces cerevisiae Cdc6 protein is crucial for DNA replication. In the absence of cyclin-dependent kinase (CDK) activity, Cdc6 binds to replication origins, and loads Mcm proteins. In the presence of CDK activity, Cdc6 does not bind to origins, and this helps prevent rereplication. CDK activity affects Cdc6 function by multiple mechanisms: CDK activity affects transcription of CDC6, degradation of Cdc6, nuclear import of Cdc6, and binding of Cdc6 to Clb2. Here we examine some of these mechanisms individually. We find that when Cdc6 is forced into the nucleus during late G1 or S, it will not substantially reload onto chromatin no matter whether its CDK sites are present or not. In contrast, at a G2/M nocodazole arrest, Cdc6 will reload onto chromatin if and only if its CDK sites have been removed. Trace amounts of nonphosphorylatable Cdc6 are dominant lethal in strains bearing nonphosphorylatable Orc2 and Orc6, apparently because of rereplication. This synthetic dominant lethality occurs even in strains with wild-type MCM genes. Nonphosphorylatable Cdc6, or Orc2 and Orc6, sensitize cells to rereplication caused by overexpression of various replication initiation proteins such as Dpb11 and Sld2. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.molbiolcell.org/content/18/4/1324.full.pdf |
| PubMed reference number | 17267692v1 |
| Volume Number | 18 |
| Issue Number | 4 |
| Journal | Molecular biology of the cell |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CDC6 protein, human Cell Nucleus Cyclin-Dependent Kinases Cyclins DNA Replication National origin Nocodazole Nuclear Import ORC6 gene Replication Initiation Replication Origin Saccharomyces cerevisiae Transcription Initiation chromatin binding cyclin-dependent protein kinase activity |
| Content Type | Text |
| Resource Type | Article |
