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Label-free biomarker sensing in undiluted serum with suspended microchannel resonators.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Muhlen, Marcio G. Von Brault, Norman D. Knudsen, Scott M. Jiang, Shaoyi Manalis, Scott R. |
| Copyright Year | 2010 |
| Abstract | Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO(2) surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/ac9027356 |
| PubMed reference number | 20148583 |
| Journal | Medline |
| Volume Number | 82 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://dspace.mit.edu/openaccess-disseminate/1721.1/67036 |
| Alternate Webpage(s) | http://manalis-lab.mit.edu/publications/marcio_ANLCHEM_2010.pdf |
| Alternate Webpage(s) | http://dspace.mit.edu/bitstream/handle/1721.1/67036/Manalis-2010-Label-Free%20Biomarker%20Sensing%20in%20Undiluted%20Serum.pdf;sequence=1 |
| Alternate Webpage(s) | https://doi.org/10.1021/ac9027356 |
| Journal | Analytical chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
