NDLI: Single B-cell Cultivation Method Cross Reference to Related Applications

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Single B-cell Cultivation Method Cross Reference to Related Applications

Content Provider	Semantic Scholar
Copyright Year	2017
Abstract	(71) Applicant: Hoffmann-La Roche Inc., Nutley, NJ (51) Int. Cl. (US) CI2N5/0781 (2006.01) (52) U.S. Cl. (72) Inventors: Josef Endl, Weilheim (DE); Natalie CPC .................................... CI2N5/0635 (2013.01) Schuhmacher, Oberhausen/Rheinhausen USPC ........................................... 435/373; 435/325 (DE); Sonja Offner, Penzberg (DE); Josef Platzer, Geretsried (DE); Basile (57) ABSTRACT Siewe, Chicago, IL (US); Irmgard Thorey, Weilheim (DE) Herein is reported a method for obtaining a B-cell comprising the following steps a) labeling B-cells, b) depositing the (73) Assignee: HOFFMANN-LA ROCHE INC., labeled B-cells as single cells, c) co-cultivating the single cell Nutley, NJ (US) deposited B-cells with feeder cells, d) selecting a B-cell pro liferating and secreting IgG in step c) and thereby obtaining a (21) Appl. No.: 13/686,538 B-cell. The labeling can be of IgG"CD19"-B-cells, IgG" CD38"-B-cells, IgG"CD268"-B-cells, IgGCD138"-B-cells, (22) Filed: Nov. 27, 2012 CD27CD138-B-cells or CD3CD27-B-cells. The method O O can comprise the step of incubating said B-cells at 37°C. for Related U.S. Application Data one hour in EL-4B5 medium prior to the depositing step. The (63) Continuation of application No. PCT/EP2011/ method can also comprise the step of centrifuging said single 058616, filed on May 26, 2011. cell deposited B-cells prior to the co-cultivation. In the co cultivation a feeder mix comprising interleukin-1beta, and (30) Foreign Application Priority Data tumor necrosis factor alpha and Staphylococcus aureus Strain
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Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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