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Hepatitis B virus core protein variations differ in tumor and adjacent nontumor tissues from patients with hepatocellular carcinoma.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhu, Yu Jin, Yingyu Cai, Xiaojin Bai, Xin Chen, Min Chen, Taoyang Wang, Jinbing Qian, Gengsun Gu, Jianren Li, Jinjun |
| Copyright Year | 2012 |
| Abstract | OBJECTIVES To characterize the mutation pattern of a hepatitis B virus (HBV) core protein (HBcAg) derived from hepatocellular carcinoma (HCC) and adjacent nontumor tissues. METHODS HBV core gene fragments (nt. 1901-2365) were amplified from 98 HBV-related HCC tissues and 33 adjacent nontumor tissues. The deduced amino acids (AAs) of the core gene were aligned with the prototype sequences of HBV genotypes B and C. RESULTS In total, there were 54 positions that showed polymorphism at the deduced AA level. The mutations were predominantly located in three major (codons 83-87, 95-104 and 130-135) and three minor (codons 21-38, 59-63 and 151-155) mutation-clustering regions (MCRs). The substitution rate in MCRs was significantly higher than in mutation-devoid regions (p < 0.001). The most frequently occurring mutations in rank were codon P130T (38.8%), I97L (37.8%) and S87G (23.5%). In addition, there were 7 patients that showed internal deletions in the middle of HBcAg with sizes ranging from 34 to 59 AAs. Unexpectedly, the core genes isolated from tumor tissues had fewer mutations compared with those isolated from adjacent nontumor tissues from the same patients (p < 0.05). CONCLUSIONS Accumulation of naturally occurring mutations in certain restricted segments of HBcAg may be related to the development of HCC. |
| Starting Page | 29 |
| Ending Page | 35 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.karger.com/Article/Pdf/322832 |
| PubMed reference number | 21325784v1 |
| Alternate Webpage(s) | https://doi.org/10.1159/000322832 |
| DOI | 10.1159/000322832 |
| Journal | Intervirology |
| Volume Number | 55 |
| Issue Number | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alignment Amino Acids Body tissue Codon (nucleotide sequence) Fifty Nine Genotype Hepatitis A Hepatitis B Core Antigen Hepatitis B Virus Hepatitis C virus Herpesvirus 1, Cercopithecine Liver carcinoma Mutation Neoplasms Patients statistical cluster |
| Content Type | Text |
| Resource Type | Article |
