NDLI: Characterization of novel esterases in insecticide-resistant mosquitoes.

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Characterization of novel esterases in insecticide-resistant mosquitoes.

Content Provider	Semantic Scholar
Author	Cui, Feng Weill, Mylène Berthomieu, Arnaud Raymond, Michel Qiao, Chuan-Ling
Copyright Year	2007
Abstract	In the mosquito Culex pipiens complex (Diptera: Culicidae), the amplification of carboxylesterase genes is an important mechanism providing resistance to organophosphate insecticides. Various amplified alleles at the Ester locus have been identified over the world. In this study, two newly detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively. A high molecular identity is observed both at the nucleotide level and at the deduced amino acid level among the known Ester alleles. Real-time quantitative PCR results suggest 2.5-fold amplification of the Ester(B10) allele, 36.5-fold amplification of the Ester(A11) allele, and 19.1-fold amplification of the Ester(B11) allele. The ca. 2-fold difference in amplification level between Ester(A11) and Ester(B11) may indicate a new model for the esterase amplification. Bioassays show that these two resistant Ester alleles only can confer moderate or low resistance to the tested organophosphate insecticides.
Starting Page	1
Ending Page	5
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://evolutionhumaine.fr/michel/publis/pdf/cui_2007_insect_biochem_mol_biol.pdf
PubMed reference number	17916499v1
Volume Number	37
Issue Number	11
Journal	Insect biochemistry and molecular biology
Language	English
Access Restriction	Open
Subject Keyword	Alleles Amino Acids Biological Assay Culex (Genus) Culicidae Esterases Esters Insecticides Nucleotides Organophosphates Reverse Transcriptase Polymerase Chain Reaction
Content Type	Text
Resource Type	Article

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