NDLI: The Effect of Conjugation on the Competition between Internal Conversion and Electron Detachment: A Comparison between Green Fluorescent and Red Kaede Protein Chromophores.

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The Effect of Conjugation on the Competition between Internal Conversion and Electron Detachment: A Comparison between Green Fluorescent and Red Kaede Protein Chromophores.

Content Provider	Semantic Scholar
Author	Tay, Jamie Parkes, Michael A. Addison, Kiri Chan, Yohan Zhang, Lijuan Hailes, Helen C. Page, Philip C. Bulman Meech, Stephen R. Blancafort, Lluís Fielding, Helen H.
Copyright Year	2017
Abstract	Kaede, an analogue of green fluorescent protein (GFP), is a green-to-red photoconvertible fluorescent protein used as an in vivo "optical highlighter" in bioimaging. The fluorescence quantum yield of the red Kaede protein is lower than that of GFP, suggesting that increasing the conjugation modifies the electronic relaxation pathway. Using a combination of anion photoelectron spectroscopy and electronic structure calculations, we find that the isolated red Kaede protein chromophore in the gas phase is deprotonated at the imidazole ring, unlike the GFP chromophore that is deprotonated at the phenol ring. We find evidence of an efficient electronic relaxation pathway from higher-lying electronically excited states to the S1 state of the red Kaede chromophore that is not accessible in the GFP chromophore. Rapid autodetachment from high-lying vibrational states of S1 is found to compete efficiently with internal conversion to the ground electronic state.
Starting Page	765
Ending Page	771
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1021/acs.jpclett.7b00174
PubMed reference number	28124921
Journal	Medline
Volume Number	8
Issue Number	4
Alternate Webpage(s)	http://discovery.ucl.ac.uk/1539958/1/kaede_jpclett_revised.pdf
Alternate Webpage(s)	https://ueaeprints.uea.ac.uk/62224/1/Accepted_manuscript.pdf
Alternate Webpage(s)	https://doi.org/10.1021/acs.jpclett.7b00174
Journal	The journal of physical chemistry letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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