Loading...
Please wait, while we are loading the content...
Similar Documents
The distribution of catecholamines, glutamate decar☐ylase and choline acetyltransferase in layers of the rat olfactory bulb
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jaffe´, E. H. Cuello, Augusto Claudio |
| Copyright Year | 1980 |
| Abstract | The mammalian olfactory bulb is a highly simplified cortical structure. It is organized in well-defined layers representing the main synaptic fields for their diverse neural elements. The synaptology of the mammalian olfactory bulb has been well established 2,2°-23,27. In this study we have attempted to correlate the distribution of the catecholamines and enzymatic markers for the chotinergic (choline acetyltransferase) and the GABAergic (glutamate decarboxylase) elements with the synaptic organization of this neural structure. For this, the combination of microdissected procedures and highly sensitive radiometric assays was applied. Male Sprague-Dawley rats of 200-250 g were used in this study. Tissue samples were obtained following a modification of the Zigmond and Ben Ari 3° technique in which the sections were not stained and the neural structures recognized under a microscope by their differential transparency under transmitted illumination 3. The following operations were carried out in a cold room. Rats under anaesthesia (Equithesin 1 ml/150 g) were perfused through the left ventricle of the heart with icecold Krebs-bicarbonate. After removal of the brain from the skull, the olfactory bulb was sliced with a Mcllwain tissue chopper into 300 #m thick sections and these were placed onto a glass slide and kept on ice. The olfactory bulb slices were examined under a dissecting microscope provided with a cool stage and divided by free-hand microdissection into 5 layers, i.e. olfactory nerve layer (ONL), glomerular layer (GL), combined external plexiform layer (EPL) and mitral cell body layer (MBL), granule cell layer (GRL) and medullar layer or olfactory tract (ML), (Fig. 1). As a control, the contralateral complete olfactory bulb was frozen directly after removal from the skull. The microdissected samples and the complete olfactory bulb were immediately assayed for catecholamine content, glutamate decarboxylase and choline acetyltransferase activity. For the assay of catecholamine content, the tissue samples were homogenized in distilled water (1:20, w/v) and samples taken for protein deter- |
| Starting Page | 232 |
| Ending Page | 237 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 7357447 |
| Journal | Medline |
| Volume Number | 186 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0006899380902723 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0006899380902723?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0006-8993%2880%2990272-3 |
| Journal | Brain Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
