NDLI: Abundance of Antibiotic Resistance Genes in Two Municipal Wastewater Treatment Plants and Receiving Water in Atlantic Canada

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Abundance of Antibiotic Resistance Genes in Two Municipal Wastewater Treatment Plants and Receiving Water in Atlantic Canada

Content Provider	Semantic Scholar
Author	McConnell, Mandy M.
Copyright Year	2017
Abstract	Wastewater treatment plants (WWTPs) constitute a reservoir for high levels of antibiotic resistance genes (ARGs), creating public health concerns due to their presence and possible transfer between bacteria. Therefore, WWTPs may represent a critical control point in reducing the release of antibiotic resistance into the environment. The objective of this study was to investigate how the abundance of ARGs is affected by individual treatment steps in two different municipal WWTPs, located in Atlantic Canada, which use two different biological treatment technologies namely aerated lagoons (ALs) versus biological nutrient removal (BNR) reactors. Quantitative real-time PCR (qPCR) was used to quantify the absolute (copies/mL) and relative abundances (copies/copies 16S rRNA) for 9 ARGs as well as one indicator integron gene (int1) to assess ARG cassette presence and ARG mobility potential. Water quality assessment revealed both plants were meeting provincial effluent regulations, however eight out of ten genes (int1, sul1, sul2, tetO, ermB, blaCTX-M, blaTEM, qnrS) were detected in all samples at both plants (vanA was not detected in any samples, and mecA was only detected above the limit of detection in winter samples at AL plant). Although absolute ARG abundances were significantly (p<0.05) decreased from influent to effluent water samples at both plants, the relative abundance remained unchanged at the AL plant and decreased slightly at the BNR plant, suggesting that the BNR system may be better equipped to decrease relative ARG
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://dalspace.library.dal.ca/bitstream/handle/10222/72949/McConnell-Mandy-MASc-ENVE-March-2017.pdf?isAllowed=y&sequence=1
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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