NDLI: Removal of Antibiotic Resistance Genes at Two Conventional Wastewater Treatment Plants of Louisiana, USA

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Removal of Antibiotic Resistance Genes at Two Conventional Wastewater Treatment Plants of Louisiana, USA

Content Provider	MDPI
Author	Thakali, Ocean Brooks, John P. Shahin, Shalina Sherchan, Samendra P. Haramoto, Eiji
Copyright Year	2020
Description	Wastewater treatment plants (WWTPs) represent all known types of antibiotic resistance mechanisms and are considered as the critical points for the spread of antibiotic resistance genes (ARGs). The purpose of this study is to investigate the removal of a Class 1 integrase gene (intI1) and a selected set of ARGs (blaTEM, ermF, mecA, and tetA) at two conventional WWTPs by using chlorination in Louisiana, USA. We collected 69 wastewater samples (23 each from influent, secondary effluent, and final effluent) and determined the concentrations of ARGs by using quantitative polymerase chain reaction. All tested ARGs, except for mecA, were detected in 83–96% and 30–65% of influent and final effluent samples, respectively. Although the ARGs underwent approximately 3-log10 reduction, two WWTPs on an average still released 3.3 ± 1.7 log10 copies/mL of total ARGs studied in the effluents. Chlorination was found to be critical in the significant reduction of total ARGs (p < 0.05). Correlation analysis and the ability of intI1 to persist through the treatment processes recommend the use of intI1 as a marker of ARGs in effluents to monitor the spread of antibiotic resistance in effluents. Our study suggests that conventional WWTPs using chlorination do not favor the proliferation of antibiotic resistance bacteria and ARGs during wastewater treatment.
Starting Page	1729
e-ISSN	20734441
DOI	10.3390/w12061729
Journal	Water
Issue Number	6
Volume Number	12
Language	English
Publisher	MDPI
Publisher Date	2020-06-17
Access Restriction	Open
Subject Keyword	Water Water Science and Technology Antibiotic Resistance Gene Chlorination Inti1 Quantitative Pcr Reduction Wastewater Treatment
Content Type	Text
Resource Type	Article

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