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Response of bacillus subtilis to antimicrobial peptide stress
| Content Provider | Semantic Scholar |
|---|---|
| Author | Höfler, Carolin |
| Copyright Year | 2016 |
| Abstract | Background: Two-component signal transduction systems are one means of bacteria to respond to external stimuli. The LiaFSR two-component system of Bacillus subtilis consists of a regular two-component system LiaRS comprising the core Histidine Kinase (HK) LiaS and the Response Regulator (RR) LiaR and additionally the accessory protein LiaF, which acts as a negative regulator of LiaRS-dependent signal transduction. The complete LiaFSR system was shown to respond to various peptide antibiotics interfering with cell wall biosynthesis, including bacitracin. Methodology and Principal Findings: Here we study the response of the LiaFSR system to various concentrations of the peptide antibiotic bacitracin. Using quantitative fluorescence microscopy, we performed a whole population study analyzed on the single cell level. We investigated switching from the non-induced ‘OFF’ state into the bacitracin-induced ‘ON’ state by monitoring gene expression of a fluorescent reporter from the RR-regulated liaI promoter. We found that switching into the ‘ON’ state occurred within less than 20 min in a well-defined switching window, independent of the bacitracin concentration. The switching rate and the basal expression rate decreased at low bacitracin concentrations, establishing clear heterogeneity 60 min after bacitracin induction. Finally, we performed time-lapse microscopy of single cells confirming the quantitative response as obtained in the whole population analysis for high bacitracin concentrations. Conclusion: The LiaFSR system exhibits an immediate, heterogeneous and graded response to the inducer bacitracin in the exponential growth phase. Citation: Kesel S, Mader A, Höfler C, Mascher T, Leisner M (2013) Immediate and Heterogeneous Response of the LiaFSR Two-Component System of Bacillus subtilis to the Peptide Antibiotic Bacitracin. PLoS ONE 8(1): e53457. doi:10.1371/journal.pone.0053457 Editor: Tarek Msadek, Institut Pasteur, France Received August 31, 2012; Accepted November 30, 2012; Published January 11, 2013 Copyright: 2013 Kesel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Funding to ML, the authors thank excellence cluster Nano Initiative Munich and the Center for Nanoscience for funding. Funding to TM, funding is gratefully acknowledged by the Deutsche Forschungsgemeinschaft (MA2873/3-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: madeleine.leisner@physik.uni-muenchen.de |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://edoc.ub.uni-muenchen.de/19073/1/Hoefler_Carolin.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
