NDLI: Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment

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Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment

Content Provider	MDPI
Author	Pang, Yaru Wu, Ruobin Cui, Tianlin Zhang, Zequn Dong, Li Chen, Fang Hu, Xiaosong
Copyright Year	2022
Description	In this study, a method of Bacillus subtilis spore inactivation under high pressure (P, 200 MPa) combined with moderate temperature (T, 80 °C) and the addition of antimicrobial peptide LK $(10^{2}$ μg/mL) was investigated. Spores presented cortex hydrolysis and inner membrane (IM) damage with an 8.16 log reduction in response to treatment with PT-LK, as observed by phase-contrast and inverted fluorescence microscopy and flow cytometry (FCM) analysis. Furthermore, a tandem mass tag (TMT) quantitative proteomics approach was utilized because Liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis data were used. After treatment with PT-LK, 17,017 polypeptides and 3166 proteins were detected from B. subtilis spores. Among them, 78 proteins showed significant differences in abundance between the PT-LK-treated and control groups, with 49 proteins being upregulated and 29 proteins being downregulated in the PT-LK-treated group. Genetic information processing, metabolism, cellular process, and environmental information processing were the main mechanisms of PT-LK-mediated spore inactivation.
Starting Page	1123
e-ISSN	23048158
DOI	10.3390/foods11081123
Journal	Foods
Issue Number	8
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-04-13
Access Restriction	Open
Subject Keyword	Foods Biochemical Research Bacillus Subtilis Spores High-pressure Processing Random Antimicrobial Peptide Mixtures Food Sterilization Spore Inactivation Proteomic
Content Type	Text
Resource Type	Article

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