NDLI: Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results.

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Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results.

Content Provider	Semantic Scholar
Author	Benkoël, Liliane Biagini, Philippe Dodero, Franck Lamballerie, Xavier De Micco, Philippe De Chamlian, Albert
Copyright Year	2004
Abstract	We localized HCV C-100 protein in liver biopsies of 15 patients with chronic hepatitis C using immunohistochemistry. The results were compared to serum, tissue extract analysis of HCV RNA and in situ RT-PCR described in a previous study. HCV was detected in 80% of the sera tested, in 40% of the tissue extracts and in 80% and 60% of the tissue sections tested by immunohistochemistry and in situ RT-PCR respectively. Compared to the serum positive cases, 83% and 67% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR and 41% were positive with tissue extract detection. Compared to the tissue extract positive cases, 25% and 50% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR. Finally, 75% of the cases positive by immunohistochemistry were also positive by in situ RT-PCR. These results underline the complementarity of the different methods for the precise diagnosis of hepatitis C.
Starting Page	49
Ending Page	57
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ejh.it/index.php/ejh/article/download/886/1001
PubMed reference number	15208088v1
Volume Number	48
Issue Number	2
Journal	European journal of histochemistry : EJH
Language	English
Access Restriction	Open
Subject Keyword	Antigens, Viral Biopsy Chemical and Drug Induced Liver Injury Eighty Embedding Formaldehyde HCV viral load Hepatitis B Hepatitis C virus Hepatitis C, Chronic Hepatitis, Chronic Liver parenchyma Patients RNA Reverse Transcriptase Polymerase Chain Reaction Tissue Extracts
Content Type	Text
Resource Type	Article

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