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Improved current methods for amplification of DNA from routinely processed liver tissue by PCR.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lamballerie, Xavier De Chapel, Françoise Zandotti, Christine |
| Copyright Year | 1994 |
| Abstract | With both a classic DNA preparation protocol (including removal of paraffin wax and protein digestion) and a DNA extraction protocol with Chelex 100, the hepatitis B virus genome was searched for using the polymerase chain reaction (PCR) in 30 samples of paraffin wax embedded liver tissue from patients with chronic hepatitis. The classic protocol was more sensitive than the rapid Chelex 100 procedure (10 v six positive samples). A third protocol, including removal of paraffin wax, protein digestion, and Chelex 100 treatment of the digestion solution before PCR, was more sensitive than the others (16 positive samples). It is concluded that it could therefore be helpful for PCR analysis of paraffin wax embedded liver tissue. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcp.bmj.com/content/jclinpath/47/5/466.full.pdf |
| PubMed reference number | 8027403v1 |
| Volume Number | 47 |
| Issue Number | 5 |
| Journal | Journal of clinical pathology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chemical and Drug Induced Liver Injury Embedding Gene Amplification Technique Hepatitis B Virus Hepatitis, Chronic Herpesvirus 1, Cercopithecine Liver parenchyma Paraffin Patients Protein Digestion (research activity) Reverse Transcriptase Polymerase Chain Reaction Waxes chelex |
| Content Type | Text |
| Resource Type | Article |
