NDLI: Interaction between Epstein-Barr virus-determined nuclear antigen (EBNA) and the viral DNA.

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Interaction between Epstein-Barr virus-determined nuclear antigen (EBNA) and the viral DNA.

Content Provider	Semantic Scholar
Author	Gergely, Lajos Luka, Janos Klein, George
Copyright Year	1984
Abstract	Epstein-Barr virus (EBV) nuclear antigen (EBNA) was purified from the Burkitt lymphoma line Raji and its EBV DNA-binding properties were characterized. EBNA binding protected fragments of about 30 bp of B95-8 cell-derived EBV DNA from an excess of DNase I. Human anti-EBNA antibodies prevented DNA binding. Purified extracts from EBNA-negative cells did not protect EBV DNA against DNase I digestion. Mapping of the EBV DNA fragments protected from endonuclease (EcoRI, HindIII, SalI) digestion revealed many binding sites. Similar results were obtained following mixing of crude cell extracts and HindIII-digested fragments of EBV DNA and subsequent immunoprecipitation of the EBNA-DNA complex. In experiments involving the analysis of EBV DNA, fragments were protected from DNase I digestion by purified EBNA.
File Format	PDF HTM / HTML
DOI	10.1099/0022-1317-65-11-2043
PubMed reference number	6094716
Journal	Medline
Volume Number	65
Part	11
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/jgv/65/11/JV0650112043.pdf?accname=guest&checksum=8116AA54EDA8A7A64B357C2D59D60342&expires=1546858830&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/65/11/JV0650112043.pdf?accname=guest&checksum=B7426DF3A62E37EC1F5BAFC968DA3E00&expires=1544090627&id=id
Journal	The Journal of general virology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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