NDLI: Epstein-Barr viral nuclear antigen 1 antisense oligodeoxynucleotide inhibits proliferation of Epstein-Barr virus-immortalized B cells

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Epstein-Barr viral nuclear antigen 1 antisense oligodeoxynucleotide inhibits proliferation of Epstein-Barr virus-immortalized B cells

Content Provider	Scilit
Author	Roth, G. Curiel, T. Lacy, J.
Copyright Year	1994
Description	The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is a latent viral protein that is expressed in all EBV-immortalized lymphocytes and plays an essential role in immortalization y EBV. EBNA-1 protein is required for replication and maintenance of the episomal viral genome in latently infected, immortalized cells. Given the essential function of EBNA-1 in immortalization, we have examined the effect of EBNA-1 antisense oligodeoxynucleotides on expression of EBNA-1 protein and proliferation in EBV-immortalized lymphoblastoid cells. We have shown that exposure to unmodified antisense oligodeoxynucleotide of codons 6 through 10 of EBNA-1 partially suppressed EBNA-1 protein expression in EBV-immortalized lymphoblastoid cells relative to untreated cells or cells exposed to two scrambled sequences of the EBNA-1 antisense. Furthermore, EBNA-1 antisense inhibited proliferation of EBV- immortalized cells by at least 50% compared with the scrambled antisense sequences. There was no difference in the effect of antisense and scrambled antisense oligodeoxynucleotides on the proliferation of EBV-negative cells, indicating that the antiproliferative effect of EBNA-1 antisense was EBV-specific. These findings underscore the essential role of EBNA-1 in immortalization and, furthermore, have potential therapeutic implications for EBV-associated neoplastic diseases.
Related Links	https://ashpublications.org/blood/article-pdf/84/2/582/230861/582.pdf
Ending Page	587
Page Count	6
Starting Page	582
DOI	10.1182/blood.v84.2.582.bloodjournal842582
Journal	Blood
Issue Number	2
Volume Number	84
Language	English
Publisher	American Society of Hematology
Publisher Date	1994-07-15
Access Restriction	Open
Subject Keyword	Virology Protein Virus Antigen Ebna Ebv Antisense Epstein Scrambled Immortalized Inhibits Proliferation Journal: Blood (Vol- 56, Issue- 2)
Content Type	Text
Resource Type	Article

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