NDLI: Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing  of a pre-mRNA Target

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Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing  of a pre-mRNA Target

Content Provider	Semantic Scholar
Author	Stepanov, G. A. Semenov, D. V. Kuligina, E. V. Koval, Oksana A. Rabinov, I. V. Kit, Y. Y. Richter, Vorsitzender
Copyright Year	2012
Abstract	Small nucleolar RNAs (snoRNAs) play a key role in ribosomal RNA (rRNA) biogenesis. Box C/D snoRNAs guide the site-specific 2'-O-ribose methylation of nucleotides in rRNAs and small nuclear RNAs (snRNAs). A number of box C/D snoRNAs and their fragments have recently been reported to regulate post-transcriptional modifications and the alternative splicing of pre-mRNA. Artificial analogues of U24 snoRNAs directed to nucleotides in 28S and 18S rRNAs, as well as pre-mRNAs and mature mRNAs of human heat shock cognate protein (hsc70), were designed and synthesized in this study. It was found that after the transfection of MCF-7 human cells with artificial box C/D RNAs in complex with lipofectamine, snoRNA analogues penetrated into cells and accumulated in the cytoplasm and nucleus. It was demonstrated that the transfection of cultured human cells with artificial box C/D snoRNA targeted to pre-mRNAs induce partial splicing impairments. It was found that transfection with artificial snoRNAs directed to 18S and 28S rRNA nucleotides, significant for ribosome functioning, induce a decrease in MCF-7 cell viability.
Starting Page	32
Ending Page	41
Page Count	10
File Format	PDF HTM / HTML
PubMed reference number	22708061
Volume Number	4
Journal	Acta naturae
Language	English
Access Restriction	Open
Subject Keyword	Alternative Splicing Cell Nucleus Cell Survival Heat-Shock Response Lipofectamine Nucleotides RNA Splicing Ribose Ribosomal RNA Ribosomes SNORD24 gene Small Nucleolar RNA Transcription, Genetic mRNA Precursor negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Content Type	Text
Resource Type	Article

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