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SR proteins: a conserved family of pre-mRNA splicing factors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zahler, Alan M. Lane, William S. Stolk, John A. Roth, Mark B. |
| Copyright Year | 1992 |
| Abstract | We demonstrate that four different proteins from calf thymus are able to restore splicing in the same splicing-deficient extract using several different pre-mRNA substrates. These proteins are members of a conserved family of proteins recognized by a monoclonal antibody that binds to active sites of RNA polymerase II transcription. We purified this family of nuclear phosphoproteins to apparent homogeneity by two salt precipitations. The family, called SR proteins for their serine- and arginine-rich carboxy-terminal domains, consists of at least five different proteins with molecular masses of 20, 30, 40, 55, and 75 kD. Microsequencing revealed that they are related but not identical. In four of the family members a repeated protein sequence that encompasses an RNA recognition motif was observed. We discuss the potential role of this highly conserved, functionally related set of proteins in pre-mRNA splicing. |
| Starting Page | 1 |
| Ending Page | 13 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://genesdev.cshlp.org/content/6/5/837.full.pdf |
| PubMed reference number | 1577277v1 |
| Volume Number | 6 |
| Issue Number | 5 |
| Journal | Genes & development |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Sequence Arginine Carboxyl Group Monoclonal Antibodies Phosphoproteins Protein Domain RNA Polymerase II RNA Splicing Factors mRNA Precursor negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Content Type | Text |
| Resource Type | Article |
