NDLI: Analysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)

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Analysis of carotenoid accumulation and expression of carotenoid biosynthesis genes in different organs of Chinese cabbage (Brassica rapa subsp. pekinensis)

Content Provider	Semantic Scholar
Author	Tuan, Pham Anh Kim, Jae Kwang Lee, Jeongyeo Park, Woo Tae Kwon, Do Yeon Kim, Yeon Bok Kim, Haeng Hoon Park, Sang Un
Copyright Year	2012
Abstract	The relationship between carotenoid accumulation and expression of carotenoid biosynthesis genes was investigated in the flowers, stems, young leaves, old leaves, and roots of Chinese cabbage (Brassica rapa subsp. pekinensis). Quantitative real-time PCR analysis showed that the mRNA levels of BrPSY, BrPDS, BrZDS, BrLCYB, BrLCYE, BrCHXB, and BrZEP leading to the production of carotenoids were highest in the flowers or the leaves and lowest in the roots of Chinese cabbage. In contrast, the mRNA expression of BrNCED, a gene involved in abscisic acid (ABA) biosynthesis, was highest in the roots. High-performance liquid chromatography revealed that carotenoids, namely, lutein and β-carotene, were distributed predominantly in the flowers and leaves, with very little in the underground organ, the roots. Specifically, old leaves contained 120.3 μg/g lutein and 103.93 μg/g β-carotene, which is the most potent dietary precursor of vitamin A. Moreover, we found a relatively large amount of cis isomers of β-carotene, namely, 9-cis β-carotene and 13-cis β-carotene, in Chinese cabbage. These results provide insight into carotenoid biosynthetic mechanisms in Chinese cabbage and may be helpful in the metabolic engineering of carotenoid biosynthesis in plants.
Starting Page	508
Ending Page	516
Page Count	9
File Format	PDF HTM / HTML
DOI	10.17877/DE290R-4958
PubMed reference number	27540344
Journal	Medline
Volume Number	11
Alternate Webpage(s)	https://eldorado.tu-dortmund.de/bitstream/2003/29771/1/Park_22082012_proof.pdf
Journal	EXCLI journal
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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