Loading...
Please wait, while we are loading the content...
Similar Documents
Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kota, Jhansi Melin-Larsson, Monika Ljungdahl, Per Olof Forsberg, Hanna |
| Copyright Year | 2007 |
| Abstract | Nutrient uptake in the yeast Saccharomyces cerevisiae is a highly regulated process. Cells adjust levels of nutrient transporters within the plasma membrane at multiple stages of the secretory and endosomal pathways. In the absence of the ER-membrane-localized chaperone Shr3, amino acid permeases (AAP) inefficiently fold and are largely retained in the ER. Consequently, shr3 null mutants exhibit greatly reduced rates of amino acid uptake due to lower levels of AAPs in their plasma membranes. To further our understanding of mechanisms affecting AAP localization, we identified SSH4 and RCR2 as high-copy suppressors of shr3 null mutations. The overexpression of SSH4, RCR2, or the RCR2 homolog RCR1 increases steady-state AAP levels, whereas the genetic inactivation of these genes reduces steady-state AAP levels. Additionally, the overexpression of any of these suppressor genes exerts a positive effect on phosphate and uracil uptake systems. Ssh4 and Rcr2 primarily localize to structures associated with the vacuole; however, Rcr2 also localizes to endosome-like vesicles. Our findings are consistent with a model in which Ssh4, Rcr2, and presumably Rcr1, function within the endosome-vacuole trafficking pathway, where they affect events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.genetics.org/content/genetics/175/4/1681.full.pdf |
| PubMed reference number | 17287526v1 |
| Volume Number | 175 |
| Issue Number | 4 |
| Journal | Genetics |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Permease Amino Acids Cell secretion Endosomes Genes, Suppressor Membrane Proteins Membrane Transport Proteins Molecular Chaperones Mutation Null Value Plasma membrane Saccharomyces cerevisiae Tissue membrane Uracil Vacuole Vesicle (morphologic abnormality) amino acid import inorganic phosphate mutant physical hard work transporter activity |
| Content Type | Text |
| Resource Type | Article |
