Amino acid transporters of lower eukaryotes: regulation, structure and topogenesis.

Content Provider	Semantic Scholar
Author	Sophianopoulou, Vicky Diallinas, George
Copyright Year	1995
Abstract	Lower eukaryotes such as the yeast Saccharomyces cerevisiae and the filamentous fungus Aspergillus nidulans possess a multiplicity of amino acid transporters or permeases which exhibit different properties with respect to substrate affinity, specificity, capacity and regulation. Regulation of amino acid uptake in response to physiological conditions of growth is achieved principally by a dual mechanism; control of gene expression, mediated by a complex interplay of pathway-specific and wide-domain transcription regulatory proteins, and control of transport activities, mediated by a series of protein factors, including a kinase, and possibly, by amino acids. All fungal and a number of bacterial amino acid permeases show significant sequence similarities (33-62% identity scores in binary comparisons), revealing a unique transporter family conserved across the prokaryotic-eukaryotic boundary. Prediction of the topology of this transporter family utilizing a multiple sequence alignment strongly suggests the presence of a common structural motif consisting of 12 alpha-helical putative transmembrane segments and cytoplasmically located N- and C-terminal hydrophilic regions. Interestingly, recent genetic and molecular results strongly suggest that yeast amino acid permeases are integrated into the plasma membrane through a specific intracellular translocation system. Finally, speculating on their predicted structure and on amino acid sequence similarities conserved within this family of permeases reveals regions of putative importance in amino acid transporter structure, function, post-translational regulation or biogenesis.
Starting Page	53
Ending Page	75
Page Count	23
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsre/16/1/10.1111/j.1574-6976.1995.tb00155.x/2/16-1-53.pdf?Expires=1497102270&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=cZOyYufVxfdAn8MsvkPzS79z8imJofLxPMSdImGznRqpmKXK-~~9xN1dn0ksTFxMTz1rTbChnLS1dDeObNa5tHdVmzAoXRyWHEdi6HGhKEQ1F6ETmMN9-kAoKieETAIsUh3wqMyEcZNCbE3~jSjhRS~LCiod7ODusygVzK-14yMqoBDG38grcWNHBrdi2sYBNiOO7Zr5CCYgc0jdoFY2Cu1ozhEbmvW~aNDz33lcsVcjbdWm7siZw8ZPQK0-AkvNlB6hIvFYvRhDccZv2pn0gQx5jUOAJsIZyXj~tTEzcFwAfBy9NxJHotz02qsPUJoC0BGF~hRZP5YecGZwlx08bQ__
PubMed reference number	7888172v1
Volume Number	16
Issue Number	1
Journal	FEMS microbiology reviews
Language	English
Access Restriction	Open
Subject Keyword	Amino Acid Permease Amino Acid Sequence Amino Acid Transporter Amino Acid [EPC] Amino Acids Anatomy, Regional Dual Filamentous fungus Fungi Gene Expression Membrane Transport Proteins Plasma membrane Post-Translational Regulation Protein Domain Saccharomyces cerevisiae Sequence Alignment Translational Regulation amino acid import multiplicity
Content Type	Text
Resource Type	Article