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Solvent-selective membranes for automating sequential liquid release and routing of nucleic acid purification protocols on a simple spindle motor
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gaughran, Jennifer Kinahan, David Mishra, Rohit Ducrée, Jens |
| Copyright Year | 2016 |
| Abstract | By incorporating a set of membranes which selectively dissolve upon contact with aqueous or or-ganic solvents at strategic locations on a disc cartridge, we succeeded to fully automate the solid-phase extraction of nucleic acids by varying the spin rate of a low-cost spindle motor. A solvent- and phase-selective graphene oxide (GO) membrane governs the routing of flows to designated elution and waste chambers. The serial release of on-board sample and reagents is centrifugo-pneumatically controlled by our previously introduced, event-triggered valving scheme. The entire process of ex-traction completes in less than 8 minutes. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://doras.dcu.ie/21442/1/page0876.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |