NDLI: Solvent-selective routing for centrifugally automated solid-phase purification of RNA

Content Provider	Semantic Scholar
Author	Dimov, Nikolay Clancy, Eoin Gaughran, Jennifer Boyle, David F. Auley, Darren Mc Glynn, Macdara T. Dwyer, Róisín M. Coughlan, Helena Barry, Thomas Barrett, Louise M. Smith, Terry J. Ducrée, Jens
Copyright Year	2015
Abstract	Abstract We present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves . Using a bead-based stationary phase at the entrance of the router, we show that total RNA is purified with high integrity from cultured MCF7 and T47D cell lines, human leucocytes and Haemophilus influenzae cell lysates. Furthermore, we demonstrate the broad applicability of the device through the in vitro amplification of RNA purified on-disc using RT-PCR and NASBA. Our novel router will be at the pivot of a forthcoming, fully integrated and automated sample preparation system for RNA-based analysis .
Starting Page	859
Ending Page	871
Page Count	13
File Format	PDF HTM / HTML
DOI	10.1007/s10404-014-1477-9
Volume Number	18
Alternate Webpage(s)	https://aran.library.nuigalway.ie/bitstream/handle/10379/4668/2014_Dimov_N_et_al_Final_Submission.pdf;jsessionid=043A82F1EC840D6E0E04CABD6FF84E3C?sequence=1
Alternate Webpage(s)	https://uhra.herts.ac.uk/bitstream/handle/2299/19848/Accepted_Manuscript.pdf;jsessionid=B240ECB35101493FC4C28D7A6F5D1DD1?sequence=2
Alternate Webpage(s)	https://aran.library.nuigalway.ie/bitstream/handle/10379/4668/2014_Dimov_N_et_al_Final_Submission.pdf;jsessionid=F195557D2295755378437E75C2789C1C?sequence=1
Alternate Webpage(s)	https://aran.library.nuigalway.ie/bitstream/handle/10379/4668/2014_Dimov_N_et_al_Final_Submission.pdf;jsessionid=BB71C2EC5465BCB2ABC71897A28917F7?sequence=1
Alternate Webpage(s)	https://aran.library.nuigalway.ie/bitstream/handle/10379/4668/2014_Dimov_N_et_al_Final_Submission.pdf;jsessionid=A5EEAACE1CDAD3B53653D4292F7026F3?sequence=1
Alternate Webpage(s)	https://doi.org/10.1007/s10404-014-1477-9
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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