NDLI: GFAP expression in injured astrocytes in rats

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GFAP expression in injured astrocytes in rats

Content Provider	Semantic Scholar
Author	Zhang, Shuquan Wu, Minfei Peng, Chuangang Zhao, Guanjie Gu, Rui
Copyright Year	2017
Abstract	Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3ε by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3ε protein localization by double-labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3ε expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries.
Starting Page	1905
Ending Page	1908
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/etm/14/3/1905/download
PubMed reference number	28962102v1
Alternate Webpage(s)	https://doi.org/10.3892/etm.2017.4760
DOI	10.3892/etm.2017.4760
Journal	Experimental and therapeutic medicine
Volume Number	14
Language	English
Access Restriction	Open
Subject Keyword	Acids Astrocytes CNS disorder Cerebral cortex Glial Fibrillary Acidic Protein Glucose Hypoxia Hypoxia-Ischemia, Brain Immunofluorescence assay Ischemia Neuroglia Reperfusion Therapy Traumatic injury Western Blot intracellular protein transport
Content Type	Text
Resource Type	Article

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