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Potentialités de bioremédiation par l’utilisation des associations rhizobactéries métallo-résistantes / légumineuses : vers une nouvelle approche de rhizoremédiation des sols contaminés par les métaux
| Content Provider | Semantic Scholar |
|---|---|
| Author | Aafi, Najla El |
| Copyright Year | 2016 |
| Abstract | The aim of this work was exploring the bioremediation potential of leguminous plants and the associated rhizobacteria in order to propose a bioprocess to restore heavy metals contaminated soils of Midelt. We have isolated rhizospheric and nodules strains from root of Anthyllis vulneraria growing on contaminated soil substrates. The heavy metals resistance of all bacteria has been evaluated. The bacteria showed great heavy metals-tolerant abilities. Amplification and restriction analysis of ribosomal 16S rDNA (ARDRA) and 16S ribosomal DNA (rDNA) sequencing were used to assess biodiversity and phylogenetic position among bacteria present in nodules. Our results suggest that a great diversity of non-rhizobial bacteria colonize nodules of Anthyllis plants in contaminated soils. Also, phenotypic and molecular characterization of heavy metal tolerance was made for the strain MSMC541 isolated from the root nodules of lupine. A rhizoremediation pot experiment was conducted using Lupinus luteus grown on sand supplemented with heavy metals. The results showed that inoculation with MSMC541 improved the plant tolerance to metals. At the same time, metal translocation to the shoot was significantly reduced upon inoculation. These results suggest that Lupinus luteus plants, inoculated with the metal resistant strain Serratia sp. MSMC541, have a great potential for phytostabilization of metal contaminated soils. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://toubkal.imist.ma/bitstream/handle/123456789/10289/THESE_EL%20AAFI.pdf?sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |