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Cyclin G1 is a target of miR-122a, a microRNA frequently down-regulated in human hepatocellular carcinoma.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gramantieri, Laura Ferracin, Manuela Fornari, Francesca Veronese, Angelo Sabbioni, Silvia Liu, Chang-Gong Calin, George A. Giovannini, Catia Ferrazzí, Eros Grazi, Gian Luca Croce, Carlo M. Bolondi, Luigi Negrini, Massimo |
| Copyright Year | 2007 |
| Abstract | We investigated the role of microRNAs (miRNAs) in the pathogenesis of human hepatocellular carcinoma (HCC). A genome-wide miRNA microarray was used to identify differentially expressed miRNAs in HCCs arisen on cirrhotic livers. Thirty-five miRNAs were identified. Several of these miRNAs were previously found deregulated in other human cancers, such as members of the let-7 family, mir-221, and mir-145. In addition, the hepato-specific miR-122a was found down-regulated in approximately 70% of HCCs and in all HCC-derived cell lines. Microarray data for let-7a, mir-221, and mir-122a were validated by Northern blot and real-time PCR analysis. Understanding the contribution of deregulated miRNAs to cancer requires the identification of gene targets. Here, we show that miR-122a can modulate cyclin G1 expression in HCC-derived cell lines and an inverse correlation between miR-122a and cyclin G1 expression exists in primary liver carcinomas. These results indicate that cyclin G1 is a target of miR-122a and expand our knowledge of the molecular alterations involved in HCC pathogenesis and of the role of miRNAs in human cancer. |
| Starting Page | 2880 |
| Ending Page | 2894 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://apollo11.isto.unibo.it/Medicina/Biologia/Gramantieri,%20miR,%202007.pdf |
| Alternate Webpage(s) | http://cancerres.aacrjournals.org/content/canres/67/13/6092.full.pdf |
| PubMed reference number | 17616664v1 |
| Volume Number | 67 |
| Issue Number | 13 |
| Journal | Cancer research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cultured Cell Line Cyclin G1 Cyclins Deregulation HCCS gene Liver carcinoma MIR145 wt Allele MIR221 wt Allele Malignant Neoplasms MicroRNAs Northern Blot efmoroctocog alfa 1 UNT Injection [Eloctate] |
| Content Type | Text |
| Resource Type | Article |
