Down-regulated in Human Hepatocellular Carcinoma Cyclin G 1 Is a Target of miR-122 a , a MicroRNA Frequently

Content Provider	Semantic Scholar
Author	Gramantieri, Laura Ferracin, Manuela Fornari, Francesca Veronese, Angelo Sabbioni, Silvia Liu, Chang-Gong Calin, George Adrian Giovannini, Catia Ferrazzí, Eros Grazi, Gian Luca Croce, Carlo M. Bolondi, Luigi Negrini, Massimo
Copyright Year	2007
Abstract	We investigated the role of microRNAs (miRNAs) in the pathogenesis of human hepatocellular carcinoma (HCC). A genome-wide miRNA microarray was used to identify differentially expressed miRNAs in HCCs arisen on cirrhotic livers. Thirty-five miRNAs were identified. Several of these miRNAs were previously found deregulated in other human cancers, such as members of the let-7 family, mir-221, and mir-145 . In addition, the hepato-specific miR-122a was found downregulated in f70% of HCCs and in all HCC-derived cell lines. Microarray data for let-7a, mir-221, and mir-122a were validated by Northern blot and real-time PCR analysis. Understanding the contribution of deregulated miRNAs to cancer requires the identification of gene targets. Here, we show that miR-122a can modulate cyclin G1 expression in HCC-derived cell lines and an inverse correlation between miR-122a and cyclin G1 expression exists in primary liver carcinomas. These results indicate that cyclin G1 is a target of miR-122a and expand our knowledge of the molecular alterations involved in HCC pathogenesis and of the role of miRNAs in human cancer. [Cancer Res 2007;67(13):6092–9]
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Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/67/13/6092.full.pdf
Language	English
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Content Type	Text
Resource Type	Article