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Distributions of aspartate aminotransferase and malate dehydrogenase activities in rat retinal layers.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ross, Chris D. Godfrey, Donald A. |
| Copyright Year | 1985 |
| Abstract | Aspartate aminotransferase (AAT), an enzyme interconverting glutamate and aspartate, has been suggested to be a marker for glutamatergic and/or aspartatergic neurons. However, AAT, glutamate, and aspartate are also involved in cellular metabolism, e.g., the malate-aspartate shuttle. To investigate the extent to which AAT might be involved in these several functions in retina, the distribution of AAT activity in rat retinal layers was compared to that of malate dehydrogenase (MDH), an enzyme of aerobic metabolism proposed to be physically complexed with AAT in the malate-aspartate shuttle mechanism. The distribution of AAT activity in retinal layers closely paralleled that of MDH (correlation coefficient AAT versus MDH = 0.93). AAT activity was proportionately higher than MDH in the photoreceptor inner segments, containing a high density of mitochondria, and in the outer plexiform layer (OPL), containing photoreceptor terminals and bipolar and horizontal cell processes. The amount of total AAT activity in the inner segments related to the mitochondrial isoenzyme is almost twice that in the other layers tested, including the OPL. The correlation between AAT and MDH activities is consistent with AAT involvement in retinal energy metabolism, although other functions, such as neurotransmission, are possible. |
| Starting Page | 75 |
| Ending Page | 91 |
| Page Count | 17 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jhc.sagepub.com/content/33/7/624.full.pdf |
| PubMed reference number | 4008916v1 |
| Volume Number | 33 |
| Issue Number | 7 |
| Journal | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aspartate Transaminase Aspartic Acid Cell Respiration Energy Metabolism Glutamic Acid Isoenzymes Malate Dehydrogenase Malate-Aspartate Shuttle Pathway Metabolic Process, Cellular Ornithine-oxo-acid aminotransferase Photoreceptors Retinaldehyde Structure of outer plexiform layer of retina Synaptic Transmission Transaminases anatomical layer horizontal cell |
| Content Type | Text |
| Resource Type | Article |
