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Application of immobilized aspartate aminotransferase and immobilized aspartate aminotransferase-malate dehydrogenase coupled system to micro-assay of L-aspartic acid.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ikeda, Shu Ichi Sumi, Yusuke Fukui, Sakuzo |
| Copyright Year | 1974 |
| Abstract | Usefulness of immobilized enzymes in analyses has been well recognized [ 1,2] . Especially, immobilized multi-enzyme systems would be the most promising materials for micro-analyses of various biological compounds. Newirth et al. [3] have reported the determination of phosphoenolpyruvate by use of the combined system of immobilized pyruvate kinase and immobilized lactate dehydrogenase. Automatic determination of disaccharides has been performed by Inman and Hornby with some immobilized linked enzyme systems [4]. In previous papers [5,6], the present authors reported the preparation of immobilized tryptophanase and immobilized tryptophanase-lactate dehydrogenase coupled system and the application to the micro-assay of L-tryptophan. This paper describes the immobilization of aspartate aminotransferase (AAT) and of a coupled system of AAT and malate dehydrogenase (MDH) and their application to a micro-assay of L-aspartate. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0014-5793(74)81033-1 |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82247737.pdf |
| PubMed reference number | 4473381 |
| Alternate Webpage(s) | https://doi.org/10.1016/0014-5793%2874%2981033-1 |
| Journal | Medline |
| Volume Number | 47 |
| Issue Number | 2 |
| Journal | FEBS letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
