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Rad52 and Rad59 exhibit both overlapping and distinct functions.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Feng, Qi Düring, Louis Mayolo, Adriana Antúnez De Lettier, Gaëlle Lisby, Michael Erdeniz, Naz Mortensen, Uffe Hasbro Rothstein, Rodney |
| Copyright Year | 2007 |
| Abstract | Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination. |
| Starting Page | 27 |
| Ending Page | 37 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://orbit.dtu.dk/files/5182817/52_59.pdf |
| PubMed reference number | 16987715v1 |
| Volume Number | 6 |
| Issue Number | 1 |
| Journal | DNA repair |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chimera organism DNA Breaks, Double-Stranded DNA Breaks, Single-Stranded Providing (action) Saccharomyces cerevisiae VDJ Recombinases double-strand break repair via homologous recombination recombinase |
| Content Type | Text |
| Resource Type | Article |
